香鱼人工育苗技术

根据作者多年来香鱼人工育苗的实践经验，总结了香鱼人工繁殖和苗种培育中关键性的技术。     

芝麻的核型与系统演化

对１０份粒色不同的栽培和野生芝麻的染色体组型研究表明，栽培芝麻染色体数目一致，核型基本相同：２ｎ＝２６＝１２ｍ＋１２Ｓｍ＋２Ｓｔ随着粒色的变浅，核型的不对称性加强，有两对随体染色体。野生芝麻核型与栽培芝麻相似：２ｎ＝２６＝１２ｍ＋１２Ｓｍ（４ＳＡＴ）染色体数目有２ｎ＝２６，２ｎ＝３２，２ｎ＝５８或２ｎ＝６４．芝麻可能是由对称核型向不对称核型演化，其趋势为：野生（２ｎ＝２６）－黑粒－褐袜－黄粒－白粒     

低温热水地板供暖系统简述

本文介绍了低温热水地板供暖系统的特点。对设计中存在的几个问题进行分析:热负荷计算问题、户内系统阻力损失和地板加热盘管敷设形式及间距选择问题。     

关于《中图法》第四版现代舞、交际舞及体育舞蹈类目设置的探讨——兼与张晶老师商榷

针对现代舞、交际舞、体育舞蹈等类图书归类时出现的归类混乱的问题进行了详细分析，指出了《中图法》四版在此类类目设置的不足之处，并提出了解决问题的办法。     

现代化战争中的媒体角色——伊拉克战争中美国媒体给我们的启示

有人说战争是媒体的狂欢节，而美国媒体向来是擅长同战争“共舞”的。在伊拉克战争中美国媒体就发挥了重要作用，成为战争的一个组成部分。如今，媒体对现代化战争所起的作用越来越重要，如何在战争中充分发挥媒体的作用，值得我们认真思考。美国媒体在伊拉克战争中，扮演着一个全新的角色，这为如何在现代战争巾运用媒体留下了宝贵的经验，带给我们重要启示。     

Stress parameters and immune response of layers under different cage floor and density conditions

The aim of the experiment was to investigate the effects of cage floor and cage density on stress parameters of laying hens. A total of 162 brown laying hens (Hyline Brown), aged 34 weeks, were used in the experiment. Compact-type battery cages, with three floors, were used. Hens were allocated as one, three or five hens in each of 18 cages to obtain three different cage density groups of 1968, 656 and 393.8 cm² floor area per hen, respectively. The same number of cages with different cage density were allocated to three different battery floors (first floor=top, second=middle, third=bottom) systematically. Values for body weight, mortality rate, egg weight, egg production, egg quality characteristics, egg yolk cholesterol content, the levels of blood plasma corticosterone, serum glucose, total cholesterol and triglycerides, the ratio of heterophils to lymphocytes (H-L ratio), antibody titers, claw length score, foot health score, plumage score and throat skin injuries were taken as indicators of stress. The values for egg weight (P<0.01) at the first floor were greater than the other floor levels. The group with five hens per cage had significantly lower mean estimates (P<0.01) than other groups with respects to body weight (P<0.001), egg production (P<0.001), egg weight (P<0.001) and plumage score (P<0.01), while significantly higher mean estimates for egg albumen index (P<0.01), Haugh unit (P<0.01), serum glucose (P<0.001), and H-L ratio (P<0.001). Serum cholesterol was higher in cages with one hen than that with five hens, whereas plasma corticosterone was lower. Antibody titers in cages with one hen was similar to that with three or five hens; however, those with three hens had higher titers than those with five hens. Values for egg breaking strength, yolk index, egg cholesterol content, and foot health score were not affected by cage density or floor. The results suggest that the allocation of three hens per cage had no measurable effect on health and welfare.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β1 mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.     

Predicting the spatial distribution of ground flora on large domains using a hierarchical Bayesian model

Accomodation of important sources of uncertainty in ecological models is essential to realistically predicting ecological processes. The purpose of this project is to develop a robust methodology for modeling natural processes on a landscape while accounting for the variability in a process by utilizing environmental and spatial random effects. A hierarchical Bayesian framework has allowed the simultaneous integration of these effects. This framework naturally assumes variables to be random and the posterior distribution of the model provides probabilistic information about the process. Two species in the genus Desmodium were used as examples to illustrate the utility of the model in Southeast Missouri, USA. In addition, two validation techniques were applied to evaluate the qualitative and quantitative characteristics of the predictions.This revised version was published online in May 2005 with corrections to the Cover Date.